Part:BBa_K1937008

AF-A antifungal operon in pSBBs0K-mini

(Chassis B. subtilis, carrier plasmid pSBBs0K-mini)
Length: 6558bp
Background:
This biobrick is an assembly of the K1937001 pSBBs0K-mini B. subtilis plasmid with the K1937007 part containing AF-A (a part with two genes encoding antifungal peptides: a cutted Metchnikowin and the D4E1 peptide under the control of the strong constitutive promoter pVeg).

This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1228
  Illegal suffix found in sequence at 1256
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1228
  Illegal NheI site found at 250
  Illegal NheI site found at 1250
  Illegal SpeI site found at 1257
  Illegal PstI site found at 1271
  Illegal NotI site found at 1234
  Illegal NotI site found at 1264
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1228
  Illegal BglII site found at 970
  Illegal BglII site found at 5785
  Illegal BamHI site found at 1301
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1228
  Illegal suffix found in sequence at 1257
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1228
  Illegal XbaI site found at 1243
  Illegal SpeI site found at 1257
  Illegal PstI site found at 1271
  Illegal NgoMIV site found at 2848
  Illegal AgeI site found at 5396
  Illegal AgeI site found at 6358
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 438
  Illegal BsaI.rc site found at 1026
  Illegal BsaI.rc site found at 2672
  Illegal BsaI.rc site found at 4111
  Illegal BsaI.rc site found at 6627
  Illegal SapI site found at 1589
  Illegal SapI.rc site found at 5609